From an email:<\/p>\n
\nRemember, if you are running 15N HSQCs on double labeled protein, you should use the -LABEL_CN option in the pulse program, which incorporates 13C decoupling, and will improve the look of your HSQC in the 15N dimension, as it removes the one-bond N-C coupling\n<\/p><\/blockquote>\n
\nYou see, I recognize the words, but they make no sense to me. <\/p>\n
\nRunning through the Babelizer<\/a> improves things, I feel:<\/p>\n
\nThey call it, if 15N executed HSQCs in double noticeable protein, if
\nthat one he uses the option of LABEL_CN in the program of the
\nmovement, that account decides that 13C to him and if flied the one of
\nits HSQC in the measurement of 15N becomes the end still to improve
\nthe Vista inside ignited, from cancellations the connection an
\nobligation of GOLD<\/p>\n\n<\/p><\/blockquote>\n
\n","protected":false},"excerpt":{"rendered":"
From an email: Remember, if you are running 15N HSQCs on double labeled protein, you should use the -LABEL_CN option in the pulse program, which incorporates 13C decoupling, and will improve the look of your HSQC in the 15N dimension, … Continue reading