{"id":229,"date":"2009-02-24T11:40:54","date_gmt":"2009-02-24T11:40:54","guid":{"rendered":"http:\/\/occamstypewriter.org\/rpg\/2009\/02\/24\/on_the_last_days\/"},"modified":"2009-02-24T11:40:54","modified_gmt":"2009-02-24T11:40:54","slug":"on_the_last_days","status":"publish","type":"post","link":"https:\/\/occamstypewriter.org\/rpg\/2009\/02\/24\/on_the_last_days\/","title":{"rendered":"On the last days"},"content":{"rendered":"<p>(the title refers not to <a href=\"http:\/\/network.nature.com\/people\/rpg\/blog\/2009\/02\/23\/on-the-nature-of-faith-part-1\">this<\/a> \u2014although you might be forgiven for thinking so\u2014but to <a href=\"http:\/\/network.nature.com\/people\/rpg\/blog\/2009\/02\/16\/on-winding-down\">this<\/a>. In case you wondered.)<\/p>\n<p>\nIf you follow the instructions,<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/rg-d.com\/nature\/manuel.jpg\" alt=\"washing instructions\" width=\"1000\" height=\"552\" \/><\/p>\n<p>\nyou might get decent status.<\/p>\n<p>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/rg-d.com\/nature\/system-ok.jpg\" alt=\"upwardly mobile status\" width=\"1000\" height=\"277\" \/><\/p>\n<p>\nEven if you don&#8217;t, yesterday I split my HEKs for the last time.<\/p>\n<p>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/rg-d.com\/nature\/last-split-large.jpg\" alt=\"\" width=\"1000\" height=\"750\" \/><\/p>\n<p>\nThat&#8217;s these guys. Can you see them?<\/p>\n<p>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/rg-d.com\/nature\/last-split.jpg\" alt=\"\" width=\"403\" height=\"533\" \/><\/p>\n<p>\nCourse not. They&#8217;re not in there yet. Let&#8217;s have a look through the <a href=\"http:\/\/www.bbc.co.uk\/cult\/classic\/playschool\/\">square window<\/a>:<\/p>\n<p>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/rg-d.com\/nature\/incubator.jpg\" alt=\"\" width=\"1000\" height=\"843\" \/><\/p>\n<p>\nAnd today I performed my last (ever, probably) transfection.<\/p>\n<p>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/rg-d.com\/nature\/last-transfection.jpg\" alt=\"\" width=\"1000\" height=\"623\" \/><\/p>\n<p>\nTomorrow, or maybe Thursday, I&#8217;ll fix them in 3% paraformaldehyde (made fresh, in PBS: with 2% sucrose) and look at them under the fluorescent microscope. If it&#8217;s working. <\/p>\n<p>\nHopefully that will make <a href=\"http:\/\/www.mmb.usyd.edu.au\/mackay\/extras\/chuwedding\/DSC_2710.html\">Liza<\/a> happy.<\/p>\n<p>\n<em>sniff<\/em>. Now I&#8217;m going to cry.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>(the title refers not to this \u2014although you might be forgiven for thinking so\u2014but to this. In case you wondered.) If you follow the instructions, you might get decent status. Even if you don&#8217;t, yesterday I split my HEKs for &hellip; <a href=\"https:\/\/occamstypewriter.org\/rpg\/2009\/02\/24\/on_the_last_days\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-229","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/posts\/229","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/comments?post=229"}],"version-history":[{"count":0,"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/posts\/229\/revisions"}],"wp:attachment":[{"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/media?parent=229"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/categories?post=229"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/occamstypewriter.org\/rpg\/wp-json\/wp\/v2\/tags?post=229"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}