{"id":1610,"date":"2012-01-05T00:13:49","date_gmt":"2012-01-05T00:13:49","guid":{"rendered":"http:\/\/occamstypewriter.org\/vwxynot\/?p=1610"},"modified":"2012-01-05T00:13:49","modified_gmt":"2012-01-05T00:13:49","slug":"few-maxims-instruction-over-educated","status":"publish","type":"post","link":"https:\/\/occamstypewriter.org\/vwxynot\/2012\/01\/05\/few-maxims-instruction-over-educated\/","title":{"rendered":"A Few Maxims For The Instruction Of The Over-Educated"},"content":{"rendered":"<p><a href=\"http:\/\/en.wikipedia.org\/wiki\/Western_blot\">The Western blot<\/a> &#8211; an assay that allows you to separate all the proteins in a sample by size using <a href=\"http:\/\/en.wikipedia.org\/wiki\/Gel_electrophoresis\">gel electrophoresis<\/a> and then detect the presence of a particular protein using an antibody that (hopefully) binds specifically to it &#8211; is a staple of molecular biology. I see dozens of Westerns in an average week, and I dread to think how many I&#8217;ve seen over my whole career. However, there was a new twist in a lab meeting presentation yesterday &#8211; some of the bands (corresponding to antibody-bound proteins) were labelled as &#8220;Wilde Type&#8221;.<\/p>\n<p>This immediately brought several ideas to mind:<\/p>\n<ul>\n<li>&#8220;The importance of being transferred&#8221;<\/li>\n<li>&#8220;An ideal plus-band&#8221;<\/li>\n<li>&#8220;The only thing worse than being chemiluminesced is <em>not<\/em> being chemiluminesced&#8221;<\/li>\n<li>&#8220;One should always be a little improbe-able&#8221;<\/li>\n<li>&#8220;I have nothing to declare except my threonines&#8221;<\/li>\n<li>&#8220;Life is far too important to be taken serine-lessly&#8221;<\/li>\n<li>&#8220;To lose one control sample may be regarded as a misfortune; to lose both looks like carelessness&#8221;.<\/li>\n<li>&#8220;All postdocs become like their supervisors. That is their tragedy. No student does. That&#8217;s his&#8221;.<\/li>\n<\/ul>\n<p>Will you add your own in the comments?<\/p>\n<p>You will, readers, you will&#8230;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Western blot &#8211; an assay that allows you to separate all the proteins in a sample by size using gel electrophoresis and then detect the presence of a particular protein using an antibody that (hopefully) binds specifically to it &hellip; <a href=\"https:\/\/occamstypewriter.org\/vwxynot\/2012\/01\/05\/few-maxims-instruction-over-educated\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":10,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[26,3,25],"tags":[],"class_list":["post-1610","post","type-post","status-publish","format-standard","hentry","category-english-language","category-science","category-silliness"],"_links":{"self":[{"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/posts\/1610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/comments?post=1610"}],"version-history":[{"count":0,"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/posts\/1610\/revisions"}],"wp:attachment":[{"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/media?parent=1610"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/categories?post=1610"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/occamstypewriter.org\/vwxynot\/wp-json\/wp\/v2\/tags?post=1610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}