Typical.
I’m all excited because my paper came out, and I’m all set up to do not one, but two cutting and incisive experiments, and go into the lab on Saturday to look after the stem cells, and I find that my HEKs are clumping together.
Bah. Low grade bacterial infection. Go back on Sunday to check the split from backup, and yes, it’s still there.
If you are not a cell culturist you have no idea how depressing this is.
Today I need to remake all my media and thaw some frozen cells, so I can do these experiments. Good job we’re not going away over Christmas.
Mood: Purple
Ouch!
Don’t despair, Richard! If this can help you, I once realized that I had thrown away about 5 months of work because of a mistake in one purchase order. I was sure I was using substance X, while it turned out that I was using substance Y. And it was just my own fault.
That day, I just went home “early”. But getting back on track took far less than 5 months; I had learned quite a bit over that time anyway.
Keep it up!