Wands of DOOM

13 Responses to Wands of DOOM

1. Bob O'Hara says:

   13 October 2008 at 10:45

   If Henry’s rabbit is any guide, it’s probably a stick of celery.
2. Richard P. Grant says:

   13 October 2008 at 10:48

   I think Bob, from context, probably not.
3. Mike Fowler says:

   13 October 2008 at 11:47

   If only we could put all our work in context with the use of a simple web-link:
   Introduction:
   Science is important, particularly the area I’m interested in. For relevant background, see http:www.wikipedia.ork
   Methods: …
4. Richard P. Grant says:

   13 October 2008 at 11:56

   Hey, I’m glued, glued I tell you, to my seat.
5. Brian Clegg says:

   13 October 2008 at 12:27

   see http:www.wikipedia.ork – is this the Lord of the Rings edition?
6. Henry Gee says:

   13 October 2008 at 13:22

   Nope. Celery sticks wouldn’t be any good. I’ve tried.
7. Jennifer Rohn says:

   13 October 2008 at 16:30

   Oh, all right then:

   A Wand of Doom, earlier today
   My robot can only fill wells, not empty them. Hence I need to use suction for that purpose, and this wand is the highest throughput way I have of achieving this. As I found out to my peril last week, fly cells are a wee bit more delicate than the human carcinoma cells I normally screen, and tend to get sucked right off with the normal wand action.
   The purple tape is my attempt to pimp my wand: viz, introduce a steric hindrance so that the needles can’t get too close to the floor of the well.
   Yes, it’s a lot uglier than a solution our Italian post-doc might have crafted. But maybe slightly more complicated than cupcakes.
8. Henry Gee says:

   13 October 2008 at 16:33

   Purple-Sprouting Broccoli, anyone?
9. Richard P. Grant says:

   13 October 2008 at 20:14

   Oh my, Jenny: that’s beautiful. I’ve decided I need one.
   (Serious question: How do you sterilize it?)
10. Jennifer Rohn says:

    13 October 2008 at 20:22

    There isn’t any RNAi tissue culture protocol that requires removal of fluid from the wells, fortunately: all transfection manipulations are additive.
    The wand is used when things are no longer sterile: fixation, immunofluorence staining and washes. However, the wand is probably autoclavable, as it’s made of polypropylene and metal. But if it were me, if I had to sterilize it I’d probably do the same as I do for my robot’s tubings and needles, namely flushing it with ethanol repeatedly followed by a sterile water rinse.
11. Richard P. Grant says:

    13 October 2008 at 20:37

    Sounds like a fun Friday night.
12. Åsa Karlström says:

    13 October 2008 at 22:43

    ahh… someone else uses the purple/pinkish tape!!!
    Jenny, it looks absolutely fab! It wouldn’t have been anything like that with yellow tape… maybe the light blue… or taupe… 😉
13. Richard P. Grant says:

    15 October 2008 at 04:20

    peers
    is that Al Book in the background?

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