The Western blot – an assay that allows you to separate all the proteins in a sample by size using gel electrophoresis and then detect the presence of a particular protein using an antibody that (hopefully) binds specifically to it – is a staple of molecular biology. I see dozens of Westerns in an average week, and I dread to think how many I’ve seen over my whole career. However, there was a new twist in a lab meeting presentation yesterday – some of the bands (corresponding to antibody-bound proteins) were labelled as “Wilde Type”.
This immediately brought several ideas to mind:
- “The importance of being transferred”
- “An ideal plus-band”
- “The only thing worse than being chemiluminesced is not being chemiluminesced”
- “One should always be a little improbe-able”
- “I have nothing to declare except my threonines”
- “Life is far too important to be taken serine-lessly”
- “To lose one control sample may be regarded as a misfortune; to lose both looks like carelessness”.
- “All postdocs become like their supervisors. That is their tragedy. No student does. That’s his”.
Will you add your own in the comments?
You will, readers, you will…